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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
27/11/2018 |
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Data da última atualização: |
11/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AGUIAR, N. O.; OLIVARES, F. L.; NOVOTNY, E. H.; CANELLAS, L. P. |
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Afiliação: |
NATALIA O. AGUIAR, UENF; FABIO L. OLIVARES, UENF; ETELVINO HENRIQUE NOVOTNY, CNPS; LUCIANO P. CANELLAS, UENF. |
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Título: |
Changes in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
PeerJ, v. 6, article e5445, 2018. |
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DOI: |
https://doi.org/10.7717/peerj.5445 |
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Idioma: |
Inglês |
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Conteúdo: |
Plant growth-promoting bacteria (PGPB) and humic acids (HA) have been used as biostimulants in field conditions. The complete genomic and proteomic transcription of Herbaspirillum seropedicae and Gluconacetobacter diazotrophicus is available but interpreting and utilizing this information in the field to increase crop performance is challenging. The identification and characterization of metabolites that are induced by genomic changes may be used to improve plant responses to inoculation. The objective of this study was to describe changes in sugarcane metabolic profile that occur when HA and PGPB are used as biostimulants. Inoculum was applied to soil containing 45-day old sugarcane stalks. One week after inoculation, the methanolic extracts from leaves were obtained and analyzed by gas chromatography coupled to time-of-flight mass spectrometry; a total of 1,880 compounds were observed and 280 were identified in all samples. The application of HA significantly decreased the concentration of 15 metabolites, which generally included amino acids. HA increased the levels of 40 compounds, and these included metabolites linked to the stress response (shikimic, caffeic, hydroxycinnamic acids, putrescine, behenic acid, quinoline xylulose, galactose, lactose proline, oxyproline and valeric acid) and cellular growth (adenine and adenosine derivatives, ribose, ribonic acid and citric acid). Similarly, PGPB enhanced the level of metabolites identified in HA-treated soils; e.g., 48 metabolites were elevated and included amino acids, nucleic acids, organic acids, and lipids. Co-inoculation (HACPGPB) boosted the level of 110 metabolites with respect to non-inoculated controls; these included amino acids, lipids and nitrogenous compounds. Changes in the metabolic profile induced by HA+PGPB influenced both glucose and pentose pathways and resulted in the accumulation of heptuloses and riboses, which are substrates in the nucleoside biosynthesis and shikimic acid pathways. The mevalonate pathway was also activated, thus increasing phytosterol synthesis. The improvement in cellular metabolism observed with PGPB+HA was compatible with high levels of vitamins. Glucuronate and amino sugars were stimulated in addition to the products and intermediary compounds of tricarboxylic acid metabolism. Lipids and amino acids were the main compounds induced by co-inoculation in addition to antioxidants, stress-related metabolites, and compounds involved in cellular redox. The primary compounds observed in each treatment were identified, and the effect of co-inoculation (HACPGPB) on metabolite levels was discussed. MenosPlant growth-promoting bacteria (PGPB) and humic acids (HA) have been used as biostimulants in field conditions. The complete genomic and proteomic transcription of Herbaspirillum seropedicae and Gluconacetobacter diazotrophicus is available but interpreting and utilizing this information in the field to increase crop performance is challenging. The identification and characterization of metabolites that are induced by genomic changes may be used to improve plant responses to inoculation. The objective of this study was to describe changes in sugarcane metabolic profile that occur when HA and PGPB are used as biostimulants. Inoculum was applied to soil containing 45-day old sugarcane stalks. One week after inoculation, the methanolic extracts from leaves were obtained and analyzed by gas chromatography coupled to time-of-flight mass spectrometry; a total of 1,880 compounds were observed and 280 were identified in all samples. The application of HA significantly decreased the concentration of 15 metabolites, which generally included amino acids. HA increased the levels of 40 compounds, and these included metabolites linked to the stress response (shikimic, caffeic, hydroxycinnamic acids, putrescine, behenic acid, quinoline xylulose, galactose, lactose proline, oxyproline and valeric acid) and cellular growth (adenine and adenosine derivatives, ribose, ribonic acid and citric acid). Similarly, PGPB enhanced the level of metabolites identified in HA-treated soils; e.g., 48 meta... Mostrar Tudo |
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Palavras-Chave: |
Bactéria fixadora de nitrogênio; Bioestimulante; Promoção do crescimento vegetal; Substância húmica. |
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Thesagro: |
Biofertilizante. |
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Thesaurus Nal: |
Biofertilizers; Humic substances; Metabolomics; Nitrogen-fixing bacteria; Plant growth. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187136/1/2018-053.pdf
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Marc: |
LEADER 03533naa a2200289 a 4500
001 2100181
005 2021-11-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.7717/peerj.5445$2DOI
100 1 $aAGUIAR, N. O.
245 $aChanges in metabolic profiling of sugarcane leaves induced by endophytic diazotrophic bacteria and humic acids.$h[electronic resource]
260 $c2018
520 $aPlant growth-promoting bacteria (PGPB) and humic acids (HA) have been used as biostimulants in field conditions. The complete genomic and proteomic transcription of Herbaspirillum seropedicae and Gluconacetobacter diazotrophicus is available but interpreting and utilizing this information in the field to increase crop performance is challenging. The identification and characterization of metabolites that are induced by genomic changes may be used to improve plant responses to inoculation. The objective of this study was to describe changes in sugarcane metabolic profile that occur when HA and PGPB are used as biostimulants. Inoculum was applied to soil containing 45-day old sugarcane stalks. One week after inoculation, the methanolic extracts from leaves were obtained and analyzed by gas chromatography coupled to time-of-flight mass spectrometry; a total of 1,880 compounds were observed and 280 were identified in all samples. The application of HA significantly decreased the concentration of 15 metabolites, which generally included amino acids. HA increased the levels of 40 compounds, and these included metabolites linked to the stress response (shikimic, caffeic, hydroxycinnamic acids, putrescine, behenic acid, quinoline xylulose, galactose, lactose proline, oxyproline and valeric acid) and cellular growth (adenine and adenosine derivatives, ribose, ribonic acid and citric acid). Similarly, PGPB enhanced the level of metabolites identified in HA-treated soils; e.g., 48 metabolites were elevated and included amino acids, nucleic acids, organic acids, and lipids. Co-inoculation (HACPGPB) boosted the level of 110 metabolites with respect to non-inoculated controls; these included amino acids, lipids and nitrogenous compounds. Changes in the metabolic profile induced by HA+PGPB influenced both glucose and pentose pathways and resulted in the accumulation of heptuloses and riboses, which are substrates in the nucleoside biosynthesis and shikimic acid pathways. The mevalonate pathway was also activated, thus increasing phytosterol synthesis. The improvement in cellular metabolism observed with PGPB+HA was compatible with high levels of vitamins. Glucuronate and amino sugars were stimulated in addition to the products and intermediary compounds of tricarboxylic acid metabolism. Lipids and amino acids were the main compounds induced by co-inoculation in addition to antioxidants, stress-related metabolites, and compounds involved in cellular redox. The primary compounds observed in each treatment were identified, and the effect of co-inoculation (HACPGPB) on metabolite levels was discussed.
650 $aBiofertilizers
650 $aHumic substances
650 $aMetabolomics
650 $aNitrogen-fixing bacteria
650 $aPlant growth
650 $aBiofertilizante
653 $aBactéria fixadora de nitrogênio
653 $aBioestimulante
653 $aPromoção do crescimento vegetal
653 $aSubstância húmica
700 1 $aOLIVARES, F. L.
700 1 $aNOVOTNY, E. H.
700 1 $aCANELLAS, L. P.
773 $tPeerJ$gv. 6, article e5445, 2018.
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Registro original: |
Embrapa Solos (CNPS) |
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| Registros recuperados : 49 | |
| 6. |  | OLIVARES, F. L.; JAMES, E. K.; BALDANI, J. I.; DOBEREINER, Johanna. Microscopia otica e eletronica da interacao entre variedades de cana-de-acucar com diferentes graus de suscetibilidade e estria mosqueada e Herbaspirillum rubrisubalbicans sin. Pseudomonas rubrisubalbicans. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 29., Campo Grande, MS, ago. 1996. Fitopatologia Brasileira, Brasilia, v.21, p.344, 1996. Resumo n.59.| Biblioteca(s): Embrapa Agrobiologia. |
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| 7. | | OLIVARES, F. L.; BALDANI, V. L. D.; REIS, V. M.; BALDANI, J. I.; DOBEREINER, Johanna. Occurrence of the endophytic diazotrophs Herbaspirillum spp. in roots, stems, and leaves, predominantly of gramineae. Biology and Fertility of Soils, Berlin, v. 21, n. 3, p. 197-200, 1996.| Biblioteca(s): Embrapa Agrobiologia. |
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| 8. | | FARIA, S. M. de; LIMA, H. C. de; OLIVARES, F. L.; MELO, R. B.; XAVIER, R. Nodulacao em espécies florestais, especificidade hospedeira e implicações na sistemáitca de leguminosae. In: SIQUEIRA, J.O.; MOREIRA, F.M.S.; LOPES, A.S.; GUILHERME, L.R.G.; FAQUIN, V.; FURTINI NETO, A.E.; CARVALHO, J.G. (Ed.). Inter-Relação fertilidade, biologia do solo e nutrição de plantas. Vicosa: SBCS; Lavras: UFLA/DCS, 1999. p. 667-686.| Biblioteca(s): Embrapa Agrobiologia. |
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| 10. | | JAMES, E. K.; REIS, V. M.; OLIVARES, F. L.; BALDANI, J. I.; DOBEREINER, Johanna. Infection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus. Journal of Experimental Botany, Oxford, v. 45, n. 275, p. 757-766, 1994.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 11. | | JAMES, E. K.; OLIVARES, F. L.; BALDANI, J. I.; DOBEREINER, Johanna. Herbaspirillum, an endophytic diazotroph colonizing vascular tissue in leaves of Sorghum bicolor L. Moench. Journal of Experimental Botany, Oxford, v. 48, n. 308, p. 785-797, 1997.| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | AGUIAR, N. O.; CANELLAS, L. P.; OLIVARES, F. L.; SILVA, L. G. JR S.; NOVOTNY, E. H.; FAÇANHA, A. R. Bioatividade de ácidos húmicos extraídos de vermicomposto em diferentes estágios de maturidade. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29., 2010, Guarapari, ES. Anais... Guarapari, ES: FertBio, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 15. | | GUIMARÃES, A. P.; DINIZ, A.; CALDEIRA, E.; FAÇANHA, A.; OLIVARES, F. L.; HUERGO, L.; PEDROSA, F. O.; BODDEY, R. M. Aprimoramento da técnica purificação da nitrogenase para quantificação da FBN por abundância natural de 15N. In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 16. | | CONCEIÇÃO, P. M. da; VIEIRA, H. D.; CANELLAS, L. P.; MARQUES JÚNIOR, R. B.; OLIVARES, F. L. Recobrimento de sementes de milho com ácidos húmicos e bactérias diazotróficas endofíticas. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 4, p. 545-548, abr. 2008 Notas Científicas.
Título em inglês: Corn seed coating with humic acids and endophytic diazotrophic bacteria.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 17. | | CYSNE, A. Q.; LOPES, R.; RAIZER, M. D. M.; TOMAZ, J. S.; LOPES, M. T. G.; OLIVARES, F. L. Preparo rápido de amostras de pólen de açaí-solteiro e tucumã-do-amazonas, sem uso de acetólise, para análise em microscópio eletrônico de varredura. Manaus: Embrapa Amazônia Ocidental, 2021. 10 p. (Embrapa Amazônia Ocidental. Comunicado técnico, 151). ODS| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Amazônia Ocidental. |
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| 19. | | NOGUEIRA, E. de M.; OLIVARES, F. L.; JAPIASSU, J. C.; VILAR, C.; VINAGRE, F.; BALDANI, J. I.; HEMERLY, A. S. Characterization of glutamine synthetase genes in sugarcane genotypes with different rates of biological nitrogen fixation. Plant Science, Ireland, v. 169, n. 5, p. 819-832, nov. 2005.| Biblioteca(s): Embrapa Agrobiologia. |
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| 20. | | MARTINEZ-BALMORI, D.; SPACCINI, R.; AGUIAR, N. O.; NOVOTNY, E. H.; OLIVARES, F. L.; CANELLAS, L. P. Molecular characteristics and bioactivity of humic acids isolated from vermicomposts. In: MEETING OF THE INTERNATIONAL HUMIC SUBSTANCES SOCIETY, 17., 2014, Ioannina. Natural organic matter: structure-dynamics innovative applications: book of abstracts. Ioannina: University of Ioannina, 2014. p. 335-336.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| Registros recuperados : 49 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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